笔记详情
标题
Mice 方法学中动物描述
内容
Mice
Single-cell suspensions for FACS analysis and RNA extraction were prepared from 8- to 10-week-old female C57BL/6j mice. Transplantation studies used 8-week-old male donor C57BL/6-Ly5.1 (CD45.1; B6.SJL-Ptprca Pep3b/BoyJ) or C57BL/6-GFP mice that constitutively and ubiquitously express the enhanced green fluorescent protein (GFP) reporter gene under regulatory control of the chicken beta-actin promoter with cytomegalovirus enhancer (C57BL/6j-Tg(ACTB-EGFP)1Osb/J). Recipient mice were congenic 8- to 10-week-old female C57BL/6-Ly5.2 (CD45.2). All mice were obtained from Jackson Labs (Bar Harbor, ME) and maintained in a pathogen-free animal facility. Animal studies were approved by the Standing Committee on Animals of Harvard Medical School.
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来源
Blood. 2005 September 1; 106(5): 1574–1580.
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