ACS Appl Mater Interfaces:新型传感器可灵敏检测miRNA

2017-12-03 MedSci MedSci原创

本研究中,研究人员开发了一种基于石墨烯量子点(GQDs)和ssDNA-UCNP@SiO2的检测特定microRNA(miRNA)序列的传感器。该传感器利用GQD的sp2碳原子(主要是π-π堆积)和锚定在上转换纳米颗粒(UCNPs)上的DNA核碱基之间相互作用。这种相互作用将GQD带到ssDNA-UCNP @ SiO2系统的表面,增强了上转换发射。另一方面,锚定在上转换纳米粒子上的单链DNA(ssD


本研究中,研究人员开发了一种基于石墨烯量子点(GQDs)和ssDNA-UCNP@SiO2的检测特定microRNA(miRNA)序列的传感器。该传感器利用GQD的sp2碳原子(主要是π-π堆积)和锚定在上转换纳米颗粒(UCNPs)上的DNA核碱基之间相互作用。这种相互作用将GQD带到ssDNA-UCNP @ SiO2系统的表面,增强了上转换发射。

另一方面,锚定在上转换纳米粒子上的单链DNA(ssDNA)链与其互补的miRNA序列的杂交阻断了UCNPs通过π-π堆积与GQD相互作用的能力,由此可导致荧光强度减弱,其程度取决于miRNA序列的浓度。

研究已经证实该检测传感器的miRNA序列检测极限为10fM。

原始出处:


Marco Laurenti, Miguel Paez-Perez, et al., Enhancement of the Upconversion Emission by Visible-to-Near-Infrared Fluorescent Graphene Quantum Dots for miRNA Detection. ACS Appl Mater Interfaces. 2016 May 25; 8(20): 12644–12651. doi:  10.1021/acsami.6b02361

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    2017-12-13 smallant2002
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    2018-05-15 1e145228m78(暂无匿称)

    学习了.谢谢作者分享!

    0

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    2017-12-05 yaanren
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    2017-12-05 Homburg
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status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266659, encodeId=491e26665941, content=学习了.谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=57, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM4o2XVpJ6449yqDsjibydI5HnJ8MibGxNn4HZiaZib0bdfibbwoz3KQrIyN9AGxq36ZlZRNicYlPfOOWMtwgsajY6mGNFiaPclFdowjD0/0, createdBy=97bd1338592, createdName=清风拂面, createdTime=Mon Dec 04 07:36:00 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266644, encodeId=9efe26664422, content=学习.谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=53, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyhb9xDeHojCicsdRdyC3XXdGk2ib9yLypZuHN06WeW4HO8WN0GGrgEfvnTX8gsckof05GHOU5OM4Mvx/0, createdBy=9cee1982656, createdName=执着追梦, createdTime=Mon Dec 04 06:58:53 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266631, encodeId=b3bf26663121, content=学习了.谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4NfNLvzZhgPxRk75KAEKrLGic0S0IvH1aNynsEFQWiaNKdtzvtwj2kafCoPg2icYVbrfBCFiaS74X1uEiaaTTY1H9Xyem/0, createdBy=cec11614828, createdName=chunjianf, createdTime=Mon Dec 04 06:41:28 CST 2017, time=2017-12-04, status=1, ipAttribution=)]
    2017-12-05 mjldent
  8. [GetPortalCommentsPageByObjectIdResponse(id=1655816, encodeId=36fa165581674, content=<a href='/topic/show?id=2a011829d7' target=_blank style='color:#2F92EE;'>#ACE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=1829, encryptionId=2a011829d7, topicName=ACE)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=635024767990, createdName=shanyongle, createdTime=Sat May 05 13:23:00 CST 2018, time=2018-05-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1956663, encodeId=4032195666376, content=<a href='/topic/show?id=7647e210ac' target=_blank style='color:#2F92EE;'>#FAC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=7210, encryptionId=7647e210ac, topicName=FAC)], attachment=null, authenticateStatus=null, 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createdBy=9cee1982656, createdName=执着追梦, createdTime=Mon Dec 04 06:58:53 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266631, encodeId=b3bf26663121, content=学习了.谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4NfNLvzZhgPxRk75KAEKrLGic0S0IvH1aNynsEFQWiaNKdtzvtwj2kafCoPg2icYVbrfBCFiaS74X1uEiaaTTY1H9Xyem/0, createdBy=cec11614828, createdName=chunjianf, createdTime=Mon Dec 04 06:41:28 CST 2017, time=2017-12-04, status=1, ipAttribution=)]
    2017-12-04 清风拂面

    学习了.谢谢分享

    0

  9. 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status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266659, encodeId=491e26665941, content=学习了.谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=57, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM4o2XVpJ6449yqDsjibydI5HnJ8MibGxNn4HZiaZib0bdfibbwoz3KQrIyN9AGxq36ZlZRNicYlPfOOWMtwgsajY6mGNFiaPclFdowjD0/0, createdBy=97bd1338592, createdName=清风拂面, createdTime=Mon Dec 04 07:36:00 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266644, encodeId=9efe26664422, content=学习.谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=53, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyhb9xDeHojCicsdRdyC3XXdGk2ib9yLypZuHN06WeW4HO8WN0GGrgEfvnTX8gsckof05GHOU5OM4Mvx/0, createdBy=9cee1982656, createdName=执着追梦, createdTime=Mon Dec 04 06:58:53 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266631, encodeId=b3bf26663121, content=学习了.谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4NfNLvzZhgPxRk75KAEKrLGic0S0IvH1aNynsEFQWiaNKdtzvtwj2kafCoPg2icYVbrfBCFiaS74X1uEiaaTTY1H9Xyem/0, createdBy=cec11614828, createdName=chunjianf, createdTime=Mon Dec 04 06:41:28 CST 2017, time=2017-12-04, status=1, ipAttribution=)]
    2017-12-04 执着追梦

    学习.谢谢分享

    0

  10. 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status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266659, encodeId=491e26665941, content=学习了.谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=57, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM4o2XVpJ6449yqDsjibydI5HnJ8MibGxNn4HZiaZib0bdfibbwoz3KQrIyN9AGxq36ZlZRNicYlPfOOWMtwgsajY6mGNFiaPclFdowjD0/0, createdBy=97bd1338592, createdName=清风拂面, createdTime=Mon Dec 04 07:36:00 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266644, encodeId=9efe26664422, content=学习.谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=53, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyhb9xDeHojCicsdRdyC3XXdGk2ib9yLypZuHN06WeW4HO8WN0GGrgEfvnTX8gsckof05GHOU5OM4Mvx/0, 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    2017-12-04 chunjianf

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