Int J Dent:健康志愿者的PRP或可用于牙周炎的治疗

2019-06-20 不详 网络

本研究旨在评估健康志愿者的PRP与慢性牙周炎患者对抗牙龈卟啉单胞菌的抗菌作用是否存在差异。收集中度和重度慢性牙周炎患者的龈下菌斑以分离牙龈卟啉单胞菌。使用双离心法通过特定试剂盒收集4名患有健康牙周组织的个体和4名患有中度和重度牙周炎的患者的PRP,然后通过其最小抑制浓度(MIC)测试,粘附抗性测定和生物膜敏感性测定针对牙龈卟啉单胞菌的抗菌性质。牙龈卟啉单胞菌成功地从第21名患者的龈下斑块中分离出来

本研究旨在评估健康志愿者的PRP与慢性牙周炎患者对抗牙龈卟啉单胞菌的抗菌作用是否存在差异。

收集中度和重度慢性牙周炎患者的龈下菌斑以分离牙龈卟啉单胞菌。使用双离心法通过特定试剂盒收集4名患有健康牙周组织的个体和4名患有中度和重度牙周炎的患者的PRP,然后通过其最小抑制浓度(MIC)测试,粘附抗性测定和生物膜敏感性测定针对牙龈卟啉单胞菌的抗菌性质。

牙龈卟啉单胞菌成功地从第21名患者的龈下斑块中分离出来。在厌氧条件下孵育7-10天后,圆形,光滑和黑色菌落出现在琼脂盘中。通过MALDI-TOF进行细菌鉴定并通过PCR确认。所有测试的PRP样品显示出抑制牙龈卟啉单胞菌生长的能力。MIC值(表示为PRP的分数)为1/2,并且PRP防止牙龈卟啉单胞菌附着在盘表面上。然而,PRP对牙龈卟啉单胞菌生物膜的抑制没有强烈影响。

综上所述,该研究结果表明,具有健康牙周组织和慢性牙周炎患者的个体的PRP显示出针对牙龈卟啉单胞菌的抗菌特性。这种材料可以成为牙周治疗的辅助手段。

原始出处:

Pham TAV, Tran TTP, Luong NTM. Antimicrobial Effect of Platelet-Rich Plasma against Porphyromonas gingivalis. Int J Dent. 2019 May 13;2019:7329103. doi: 10.1155/2019/7329103. eCollection 2019.

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